In addition, synthetic peptides representing the Nt sequence of the in vivoreporter protein were used to define the oxidative and nitrosylative modifications occurring at Nt Cys in vitro.Findings of this study include the successful development of two new in vivo O2 sensor artificial N end rule substrates, in the plant genetic model Arabidopsis thaliana. A further outcome revealed that the ERFVII RELATED TO APETALA 2.12 (RAP2.12) is stabilised in the shoot and root apical meristem to a greater extent than in other regions of seedlings in response to hypoxia, indicating that meristematic cells could be important oxygen sensory zones. Cys 2 reporter proteins were used in in vivo studies to attempt to identify the nature of the Nt residues following N end rule action.
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